Anti-Ms CD11b, M1/70, AD, 100µg

A rat monoclonal IgG2b, kappa antibody from STEMCELL Technologies, Clone M1/70, is conjugated to Alexa Fluor® 488 and targets CD11b, an approximately 170 kDa type 1 transmembrane glycoprotein. CD11b associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor, which mediates processes including the adherence of neutrophils and monocytes to stimulated endothelium and the phagocytosis of complement-coated particles through interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The antibody reportedly blocks iC3b binding to Mac-1. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells; it is a relatively late marker for myeloid differentiation that is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The reagent exhibits reactivity with human, mouse, rhesus, cynomolgus, baboon, chimpanzee, and rabbit samples.

The antibody was generated using C57BL/10 mouse splenocytes as the immunogen and purified by affinity chromatography before conjugation. It is supplied in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide and is free of unconjugated Alexa Fluor® 488. Flow cytometry is the verified and reported application, and the clone has also been validated for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Mouse Monocyte Isolation Kit. For flow cytometry, the suggested use is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL, with titration recommended for optimal performance. Store undiluted at 2 - 8°C; do not freeze and protect from prolonged exposure to light. Products are for research use only.