Anti-Ms CD117, ACK2, FI, 50µg

This FITC-conjugated monoclonal antibody from STEMCELL Technologies, clone ACK2 (rat IgG2b, kappa), specifically recognises mouse CD117 (c-Kit), an approximately 145 kDa type I transmembrane receptor for c-Kit ligand (stem cell factor/steel factor) that belongs to the tyrosine kinase receptor family. CD117 is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, activating several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. It has been reported that binding of the ACK2 antibody blocks the function of CD117, and mutations in CD117 are associated with various types of tumors and the piebald trait.

The antibody was generated using mouse IL-3-dependent mast cells as the immunogen, then purified by affinity chromatography and conjugated with FITC under optimal conditions; the solution is free of unconjugated FITC. It is formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Flow cytometry is the verified and reported application. For flow cytometry, the suggested use of this antibody is ≤ 0.25 μg per 1 x 10^6 cells in 100 μL.

The product is stable when stored undiluted at 2 - 8°C; do not freeze, and protect the product from prolonged exposure to light. It is supplied as a clear liquid. According to the manufacturer's safety data sheet, the chemical is not considered hazardous by the 2012 OSHA Hazard Communication Standard and is not a hazardous substance or mixture according to the Globally Harmonized System (GHS). The product is for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.