Anti-Ms CD117, ACK2, 50µg

From STEMCELL Technologies, the Anti-Mouse CD117 Antibody (Clone ACK2) is a monoclonal reagent that reacts with mouse CD117 (c-Kit), an approximately 145 kDa type I transmembrane receptor for c-Kit ligand, also known as stem cell factor or steel factor. It is intended for use in flow cytometry.

CD117 is broadly expressed on hematopoietic stem cells in the bone marrow, including pluripotent and erythroid progenitor cells, B and T lymphocyte precursors, and mast cells. The receptor belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains together with a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces receptor dimerization and autophosphorylation, activating several intracellular signaling pathways that are critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. Mutations in CD117 have been associated with various types of tumors and with the piebald trait, an autosomal dominant abnormality of pigmentation. Published reports indicate that binding of the ACK2 antibody blocks the function of CD117.

The reagent is supplied as a liquid preparation and should be stored at 2 - 8 °C.