Anti-Ms CD117, 2B8, AZ, 25µg

Rat monoclonal IgG2b antibody from STEMCELL Technologies, conjugated to APC, recognizes mouse CD117 (c-Kit), an approximately 145 kDa type I transmembrane receptor expressed on hematopoietic stem cells in bone marrow — including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors — as well as on mast cells. Clone 2B8 was raised against mouse bone marrow mast cells and is of the IgG2b, kappa isotype; it does not block binding of c-Kit ligand.

CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand induces receptor dimerization and autophosphorylation, activating signaling pathways critical for hematopoietic stem cell proliferation and differentiation. Signaling is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. Mutations in CD117 are associated with various tumors and the piebald trait, an autosomal dominant pigmentation abnormality.

The antibody is supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, and was purified by affinity chromatography; the solution is free of unconjugated APC. For flow cytometry, the suggested use is ≤ 1 µg per 1 x 10^6 cells in 100 µL volume, with titration recommended for optimal performance. The product is verified for purity assessments of cells isolated with EasySep™ kits and is stable at 2 - 8°C when stored undiluted; do not freeze and protect from prolonged light exposure. For research use only.