Anti-Hu TRA-1-81, TRA-1-81, AD, 25tests

This mouse monoclonal IgM, kappa antibody from STEMCELL Technologies targets TRA-1-81, a high-molecular-mass (> 200 kDa) glycoprotein expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey and rat ES cell lines. No immunoreactivity is observed with mouse EC, EG, ES, or iPS cells. The antibody reacts with a carbohydrate epitope associated with the keratan-sulfated transmembrane protein podocalyxin and is resistant to treatment with neuraminidase. Clone TRA-1-81 was raised against the human embryonal carcinoma cell line 2102Ep cl.2A6. The Alexa Fluor® 488-conjugated formulation has been verified for flow cytometry, with the manufacturer indicating use for ELISA, flow cytometry, immunocytochemistry, and western blotting. Verification includes labeling of human ES and iPS cells cultured in TeSR™-E8™, mTeSR™1, and TeSR™2. The product is supplied in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, purified by affinity chromatography and free of unconjugated Alexa Fluor® 488. It is stable at 2 - 8 °C when stored undiluted, should not be frozen, and should be protected from prolonged exposure to light. The suggested use is 5 μL per 1 x 10^6 cells in 100 μL volume.