Anti-Hu TRA-1-81, TRA-1-81, AD, 100tests

STEMCELL Technologies offers this mouse monoclonal antibody, Clone TRA-1-81, which recognizes a carbohydrate epitope on the TRA-1-81 glycoprotein. According to the manufacturer, this high-molecular-mass (>200 kDa) protein is expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey and rat ES cell lines, with no immunoreactivity observed on mouse EC, EG, ES, or iPS cells. The epitope is associated with a keratan-sulfated transmembrane protein identified as podocalyxin and is resistant to treatment with neuraminidase.

The antibody is of the IgM, kappa isotype and is suitable for ELISA, flow cytometry, immunocytochemistry, immunoprecipitation, immunofluorescence, immunohistochemistry, and Western blotting applications. Conjugations offered include Alexa Fluor® 488, APC, PE, and unconjugated formats. The reagent supports stem cell biology research involving human pluripotent stem cell lines.