Anti-Hu SSEA-5, 8e11, PE, 25tests

This mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies, Clone 8e11, is PE-conjugated and recognizes stage-specific embryonic antigen-5 (SSEA-5), a terminal H type-1 glycan (Fuc1-2Galβ1-3GlcNAcβ1) expressed on cells in the inner cell mass of the human blastocyst during embryogenesis and on undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells. SSEA-5 expression is rapidly down-regulated upon cellular differentiation, and the antibody can be used alongside pluripotency surface markers such as CD9 and CD90, or CD50 and CD200, to sort and remove undifferentiated teratoma-forming cells from incompletely differentiated stem cell cultures.

The antibody was raised against undifferentiated H9 human embryonic stem cells and purified by affinity chromatography before conjugation with PE under optimal conditions, leaving the solution free of unconjugated PE and unconjugated antibody. The product is supplied as a clear liquid in a phosphate-buffered solution at pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin.

It has been verified for flow cytometry (FC), immunocytochemistry (ICC), and immunofluorescence microscopy (IF) applications, with suggested use at 5 μL per 1 x 10^6 cells in 100 μL volume for FC or at 100X dilution for ICC/IF. The clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™, mTeSR™1, and TeSR™2 media, and for purity assessments of cells isolated with EasySep™ kits. The product is stable at 2 - 8°C when stored undiluted; it should not be frozen and should be protected from prolonged light exposure. According to the manufacturer's safety data sheet, the chemical is not classified as hazardous under the 2012 OSHA Hazard Communication Standard or the Globally Harmonized System.