Anti-Hu SSEA-5, 8e11, FI, 100tests

A mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies that recognizes stage-specific embryonic antigen-5 (SSEA-5), a terminal H type-1 glycan expressed on the surface of cells in the inner cell mass of the human blastocyst during embryogenesis and on undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Expression of SSEA-5 is rapidly down-regulated upon cellular differentiation. The 8e11 clone can be used together with other pluripotency surface markers such as CD9 and CD90, or CD50 and CD200, to sort and remove undifferentiated teratoma-forming cells from incompletely differentiated stem cell cultures.

Produced against undifferentiated H9 human embryonic stem cells, the antibody is purified by affinity chromatography and conjugated with FITC, and the solution is free of unconjugated FITC. It is supplied in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin. For flow cytometry, the suggested use is 5 μL per 1 × 10^6 cells in 100 μL, with titration recommended for optimal performance in each application. The clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™, mTeSR™1, and TeSR™2 media, and for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit.

Product should be stored undiluted at 2 - 8 °C; do not freeze, and protect from prolonged exposure to light.