Anti-Hu OCT4 (OCT3), 3A2A20, 100µg

From STEMCELL Technologies, this mouse monoclonal antibody (IgG2b, kappa), clone 3A2A20, targets human OCT4 (octamer-binding transcription factor 4), also known as OCT3 and OCT3/4 — an ~40 kDa homeodomain transcription factor of the POU family. It is unconjugated and was raised against a recombinant partial human OCT4 protein (amino acids 1 - 141).

OCT4 is found in undifferentiated human embryonic stem (ES), induced pluripotent stem (iPS), embryonal carcinoma (EC) and embryonic germ (EG) cells, where it binds the octamer motif (5'- ATTTGCAT-3') and partners with factors such as SOX2 to maintain pluripotency and regulate genes including FBX15, FGF-4, REX1, SOX2 and osteopontin. Expression is down-regulated upon differentiation, making it a widely used marker of pluripotency and of certain human malignant germ cell tumours. Verified applications include flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF) and Western blotting (WB), with the clone confirmed for labelling human ES and iPS cells cultured in TeSR™-E8™, mTeSR™1 and TeSR™2.

The antibody is supplied in a phosphate-buffered solution (pH 7.2) containing 0.05% sodium azide and is purified by affinity chromatography. Suggested starting concentrations are FC, ≤ 0.1 μg per 1 x 10^6 cells in 100 μL; ICC/IF, ≤ 2.5 μg/mL; WB, ≤ 1 μg/mL. Store undiluted at 2 - 8 °C; do not freeze.