Anti-Hu MUC1 (CD227), 16A, AZ, 100tests

A mouse monoclonal IgG1 antibody from STEMCELL Technologies, clone 16A, specific for human MUC1 (CD227) and conjugated to APC. The antibody reacts with human MUC1, a large (> 250 kDa) heavily glycosylated type 1 transmembrane protein expressed on the surface of most glandular and ductal epithelial cells and a variety of hematopoietic cells. A characteristic feature of the MUC1 glycoprotein is a core domain composed of a variable number of tandem repeats and multiple oligosaccharide side chains. Because the extracellular portion of MUC1 can extend beyond most cell surface proteins, it is thought to play a role in cell-cell and cell-substrate adhesion.

The protein is highly expressed by a majority of human adenocarcinomas and is associated with a poor prognosis. In the mammary gland, MUC1 is localized on the apical plasma membrane of luminal epithelial cells. Clone 16A has a higher affinity for the glycosylated form of MUC1. Alternative names for MUC1 include CD227, EMA, Episialin, Epithelial membrane antigen, HMFG antigen, MAM6, Mucin 1, PEM, and Polymorphic epithelial mucin. The antibody was raised in Mouse (C57BL/6) using Jurkat cells expressing MUC1 as the immunogen, and has an IgG1, lambda isotype.

Flow cytometry (FC) is both the verified and reported application. The antibody has been verified for quantifying airway apical epithelial cells cultured in PneumaCult™-ALI Medium at the air-liquid interface. It is supplied as a phosphate-buffered solution at pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA, and was purified by affinity chromatography before conjugation with APC. The solution is free of unconjugated APC and unconjugated antibody. For flow cytometry, the suggested use is 5 μL per 1 x 10^6 cells in 100 μL volume, with titration recommended for optimal performance in each application. Store the product undiluted at 2 - 8°C; do not freeze, and protect from prolonged exposure to light. According to the manufacturer's safety data sheet, the product is not classified as a hazardous substance or mixture under GHS, and is a clear liquid with no information available for odor.