Anti-Hu Cykrtn20, Poly, UC, 100_g

This polyclonal antibody from STEMCELL Technologies reacts with cytokeratin 20, a low molecular weight type I cytokeratin that is a major component of mature enterocytes and goblet cells. Cytokeratin 20 is expressed primarily in the gastrointestinal epithelium, urothelium, and Merkel cells and serves as a marker of gastrointestinal differentiation. The protein contributes to the structural integrity of epithelial cells and maintains intermediate filament organization in intestinal epithelia.

The reagent is a rabbit polyclonal antibody raised against a synthesized peptide derived from the C-terminal region of human cytokeratin 20, supplied unconjugated. Species reactivity has been verified for human, mouse, and rat samples. Applications verified by the manufacturer include immunocytochemistry/immunofluorescence (ICC/IF), with immunohistochemistry (IHC) and Western blotting (WB) also reported in the literature. Suggested starting dilutions are ICC/IF at 2.5 μg/mL, IHC at 1:100–1:300, and WB at 1:500–1:2000, and titration is recommended for optimal performance in each application.

Cytokeratin 20 is widely used as a marker of pancreatic and colorectal cancer and has been detected in adenocarcinomas of the colon, pancreas, stomach, and biliary tract. Its expression has also been associated with the progression and metastasis of breast cancer, suggesting it may be useful as a biomarker for breast cancer prognosis. The antibody is purified by affinity chromatography and formulated in phosphate-buffered saline containing 0.02% sodium azide, 50% glycerol, and 0.5% bovine serum albumin. It should be stored undiluted at -20°C and remains stable until the expiry date on the label. For research use only.