Anti-Hu CD90, 5E10, PS, 25tests

Manufactured by STEMCELL Technologies, this mouse monoclonal IgG1, kappa antibody (Clone 5E10) targets CD90 (Thy-1), a GPI-linked membrane glycoprotein that is N-glycosylated at two sites, giving rise to 25 - 37 kDa molecules. The reagent is conjugated with PerCP-Cy5.5, was raised against the human HEL erythroleukemia cell line, and is purified by affinity chromatography. It is supplied in an aqueous buffer containing 0.09% sodium azide and may contain carrier protein/stabilizer.

CD90 has roles in signal transduction, cell adhesion and migration, neurite outgrowth, T cell activation, tumor suppression, and inhibition of the proliferation and differentiation of hematopoietic stem cells. It is a known ligand of ß2 and ß3 integrins and upregulates synthesis of fibronectin, osteonectin, and thrombospondin. The antigen is broadly expressed, being found on human thymocytes, neurons, some glial cells, fibroblasts, activated endothelial cells, some leukemia cell lines, and a distinct subset (< 1%) of CD3+CD4+ T cells in human peripheral blood. CD90 is also expressed by small subsets of CD34+ cells in fetal liver, umbilical cord blood, bone marrow, and mobilized peripheral blood cells, and is considered an important marker for hematopoietic stem and progenitor cells.

Species reactivity is reported for human, rhesus, cynomolgus, baboon, pigtailed macaque, dog, and pig, and the antibody has been verified and reported for flow cytometry. Suggested use is ≤ 5 μL (0.125 μg) per 1 x 10^6 cells in a 100 μL volume. The clone has been verified for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human) and MesenCult™-XF Medium. Store undiluted at 2 - 8°C; do not freeze and protect the reagent from prolonged exposure to light. Products are for research use only.