Anti-Hu CD83, HB15e, 25µg

The HB15e antibody reacts with CD83, an ~45 kDa type I transmembrane glycoprotein and member of the Siglec (sialic acid-binding immunoglobulin-like lectin) protein family, containing an extracellular V-type Ig-like domain and a C-terminal cytoplasmic tail. A soluble form of CD83 has also been identified. CD83 is expressed predominantly on circulating and tissue dendritic cells, including Langerhan cells and interdigitating reticulum cells. It is expressed weakly on activated lymphocytes and may also be found on neutrophils, monocytes, and macrophages. Expression of CD83 is typically transient, except on dendritic cells, and dependent on the cell's state or maturation stage. CD83 expression correlates with upregulation of HLA class II antigen and serves as a marker for dendritic cell maturation and activation.

Its function, though ill-defined, is essential for the development and maintenance of the immune system, and evidence suggests it may be involved in cell adhesion and signaling, antigen presentation, and activation and proliferation of lymphocytes. The soluble form of CD83 appears to be immunosuppressive, blocking T cell activation and inhibiting dendritic cell maturation. The epitope on CD83 bound by the HB15e antibody reportedly overlaps with that of antibody 1E11.

This unconjugated mouse monoclonal IgG1, kappa antibody is offered by STEMCELL Technologies and was raised against human CD83-transfected African green monkey COS-7 cells. It cross-reacts with human, rhesus, cynomolgus, baboon, chimpanzee, and pigtailed macaque samples. Flow cytometry has been verified, with suggested use at ≤ 0.5 μg per 1 × 10^6 cells in 100 μL volume or 100 μL of whole blood. Reported applications also include immunohistochemistry and immunoblotting (dot blot of non-denatured protein; not Western blotting). The antibody is purified by affinity chromatography and supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. It is stable at 2 - 8°C when stored undiluted and should not be frozen.