Anti-Hu CD71, OKT9, UC, 25µg

This mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies targets human CD71 (the transferrin receptor), an ~180-190 kDa disulfide-bonded homodimer and type 2 transmembrane glycoprotein. It is supplied unconjugated and is raised against human acute lymphoblastic leukaemia cells (Clone OKT9).

CD71 is expressed at high levels on a broad range of actively proliferating cells, as well as on reticulocytes, monocytes, macrophages and marrow stromal cells. Surface levels are low on resting leukocytes but are upregulated on lymphocytes, monocytes and macrophages following antigen or mitogen stimulation, and expression is lost during differentiation of reticulocytes into mature erythrocytes. CD71 plays an essential role in cellular growth by mediating uptake of transferrin-iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin-receptor complex to the cell surface. The OKT9 antibody binds an epitope that reportedly overlaps with that of clone CY1G4 but is distinct from the ligand binding site for transferrin.

The antibody has been verified for flow cytometry and is reported for use in functional assays, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoelectron microscopy, immunoprecipitation and Western blotting. For flow cytometry, the suggested use is ≤ 0.5 μg per 1 x 10e6 cells in 100 μL volume, and titration is recommended for optimal performance in each application. The antibody was purified by affinity chromatography and is provided in an aqueous buffer containing 0.09% sodium azide. It should be stored at 2 - 8°C and not frozen.