Anti-Hu CD71, OKT9, PE, 100tests

A monoclonal antibody from STEMCELL Technologies targeting human CD71 (transferrin receptor), clone OKT9, conjugated to phycoerythrin (PE) for high-brightness flow cytometry. The reagent is verified for flow cytometry applications. CD71 is a type 2 transmembrane glycoprotein that exists as an approximately 180 - 190 kDa disulfide-bonded homodimer and mediates uptake of transferrin–iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin–receptor complex to the cell surface. It is expressed at high levels on actively proliferating cells, as well as on reticulocytes, monocytes, macrophages, and marrow stromal cells. Surface levels are low on resting leukocytes but are upregulated on lymphocytes, monocytes, and macrophages following antigen or mitogen stimulation, and expression is lost during differentiation of reticulocytes into mature erythrocytes.

The OKT9 clone binds an epitope that reportedly overlaps with that of clone CY1G4 but is distinct from the ligand-binding site for transferrin. The antibody is of mouse IgG1, kappa isotype and is intended for research use only; recommended storage is at 2–8 °C, protected from light. Beyond flow cytometry, the antibody has been used in applications including electron microscopy, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, Western blot, and functional assays. CD71 has been employed as a marker for evaluating erythroid precursors and for assessing disorders such as erythroid leukemia and myelodysplastic syndrome, and has also served as a target for drug delivery.