Anti-Hu CD71, OKT9, FI, 100tests

This mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies targets human CD71 (transferrin receptor), clone OKT9, and is conjugated to FITC for fluorescence-based detection. CD71 is an approximately 180–190 kDa disulfide-bonded homodimer and type 2 transmembrane glycoprotein expressed at high levels on a broad range of actively proliferating cells, as well as on reticulocytes, monocytes, macrophages, and marrow stromal cells.

The antibody is verified for flow cytometry applications, with a suggested use of ≤ 5 μL (0.125 μg) per 1 x 10^6 cells in 100 μL volume. It was generated using human acute lymphoblastic leukaemia cells as the immunogen, purified by affinity chromatography, and conjugated with FITC under optimal conditions; the conjugate is free of unconjugated FITC. The OKT9 clone binds an epitope that reportedly overlaps with that of antibody clone CY1G4 but is distinct from the transferrin ligand binding site.

CD71 plays an essential role in cellular growth by mediating uptake of transferrin–iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin–receptor complex to the cell surface. The product is supplied in an aqueous buffer containing 0.09% sodium azide. It is stable at 2–8°C when stored undiluted, should not be frozen, and should be protected from prolonged exposure to light. For research use only.