Anti-Hu CD71, OKT9, BT, 100µg

This biotin-conjugated antibody from STEMCELL Technologies, clone OKT9, targets the human CD71 antigen, also known as the transferrin receptor. CD71 is an ~180–190 kDa disulfide-bonded homodimer and type 2 transmembrane glycoprotein that is expressed at high levels on a broad range of actively proliferating cells, as well as on reticulocytes, monocytes, macrophages and marrow stromal cells. Surface levels are low on resting leukocytes but are upregulated on lymphocytes, monocytes and macrophages following antigen or mitogen stimulation, and expression is lost during differentiation of reticulocytes into mature erythrocytes.

CD71 mediates uptake of transferrin–iron complexes through receptor-mediated endocytosis and recycling of the apotransferrin–receptor complex to the cell surface. It has been employed as a marker for evaluating erythroid precursors and for assessing disorders such as erythroid leukemia and myelodysplastic syndrome, and has also served as a target for drug delivery. The OKT9 clone binds an epitope that reportedly overlaps with that of antibody clone CY1G4 but is distinct from the ligand binding site for transferrin. The antibody is of mouse origin with an IgG1, kappa isotype, and the clone is verified for flow cytometry applications.

Reactive species: human. Host species: mouse. Cell type of interest: erythroid cells. Conjugations offered across the product line include APC, biotin, FITC, PE and unconjugated formats. Applications include flow cytometry, Western blot, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, electron microscopy and functional assays. Research area: stem cell biology.