Anti-Hu CD45RO, UCHL1, AD, 100tests

Offered by STEMCELL Technologies, this primary antibody (clone UCHL1) targets an extracellular epitope on CD45RO, the shortest isoform of CD45. CD45RO is a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 - 240 kDa. Excision of all three exons generates the ~180-kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The antibody has been employed to identify T-cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age, and the UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.

The antibody is of mouse origin with isotype IgG2a, kappa, and is available conjugated to Alexa Fluor 488, APC, biotin, FITC, PE, PerCP-Cyanine5.5, or unconjugated. It has been validated for chimpanzee, common marmoset, and human reactive species. Applications include cell separation, electron microscopy, ELISA, flow cytometry, functional assays, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot, supporting research areas in immunology and stem cell biology.

The antibody is used to identify T cells, including CD4+ and CD8+ T cell subsets, with associated gene ID 5788.