Anti-Hu CD45RA, HI100, UC, 100µg

This mouse monoclonal IgG2b, kappa antibody from STEMCELL Technologies, clone HI100, reacts with an extracellular epitope on the CD45RA isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5, and 6 generates CD45 isoforms with molecular masses of 180 - 240 kDa; excision of exon 4 produces the ~220-kDa CD45RA isoform, expressed on naïve T cells, subsets of B cells and monocytes, and medullary thymocytes. CD45RA functions to enhance signaling through both the T cell receptor and B cell receptor. The HI100 antibody is commonly used in combination with antibodies against the CD45RO isoform to discern naïve and memory T cells and is useful for identifying the suppressor/inducer subset of CD4+ lymphocytes, with the proportion of CD45RA+ T cells typically decreasing with age.

The antibody is unconjugated, raised against CD45RA of human origin, and shows reactivity with human and chimpanzee samples. It is purified by affinity chromatography and supplied at 0.5 mg/mL in phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide. The product is stable when stored undiluted at 2 - 8°C and should not be frozen. For flow cytometry, the suggested use is ≤ 0.1 μg per 1 × 10^6 cells in 100 μL, and titration is recommended for optimal performance in each application. Verified applications include flow cytometry; reported applications include CyTOF®, FA/Blocking, FACS, ICC, IF, IHC, IP, and WB. The clone has been verified for purity assessments of cells isolated with EasySep™ Human Naïve CD4+ T Cell Isolation Kit II.

For research use only. Not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.