Anti-Hu CD45, HI30, PB, 25µg

This is a mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies, conjugated to Pacific Blue™, that recognises all isoforms of human CD45 (also known as B220, LCA, or T200). Clone HI30 was raised against full-length human CD45 protein, purified by affinity chromatography, and cross-reacts with chimpanzee CD45. It is verified for flow cytometry and is suitable for purity assessments of cells isolated with EasySep™ kits, including partial blocking that may be observed with the EasySep™ Human CD45 Depletion Kit.

CD45 is a type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase family, expressed on most hematopoietic cells except mature erythrocytes, platelets, and plasma cells. Its cytoplasmic tail carries two tyrosine phosphatase domains that modulate substrates including the Src kinases Lck and Fyn. Alternative splicing of three exons generates up to eight isoforms with molecular masses in the range of 180 - 240 kDa. Clone HI30 binds the Leukocyte Common Antigen, an extracellular region proximal to the membrane that is shared by every isoform.

The antibody is supplied as a clear liquid in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. It is stable at 2 - 8 °C when stored undiluted; the product should not be frozen and should be protected from prolonged exposure to light. For flow cytometry, STEMCELL Technologies recommends 5 µL (test sizes) or ≤ 0.5 µg (µg sizes) per 1 x 10⁶ cells in a 100 µL volume, with titration advised for each application. The reagent is not classified as hazardous under the 2012 OSHA Hazard Communication Standard, and STEMCELL Technologies' quality management system is certified to ISO 13485. The product is for research use only and is not intended for human or animal diagnostic or therapeutic use unless otherwise stated.