Anti-Hu CD45, HI30, BT, 25µg

This biotin-conjugated mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies, clone HI30, targets the human CD45 antigen (also known as B220, LCA, or T200). CD45 is a type I transmembrane glycoprotein broadly expressed on most hematopoietic cells, though expression is absent on mature erythrocytes, platelets, and plasma cells. The HI30 clone binds the leukocyte common antigen (LCA), an extracellular region proximal to the membrane that is shared by all CD45 isoforms, which range from 180–240 kDa in molecular mass. The antibody was raised against full-length human CD45 protein and reacts with human and chimpanzee samples.

The reagent is supplied at 0.5 mg/mL in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide, and has been purified by affinity chromatography prior to biotin conjugation under conditions that leave the solution free of unconjugated biotin. Flow cytometry is listed as both a verified and reported application. For flow cytometry, the manufacturer suggests ≤ 0.125 μg per 1 × 10⁶ cells in 100 μL, with titration recommended for optimal performance. The product is stable at 2–8°C when stored undiluted and should not be frozen.

According to the safety data sheet, the product is not classified as a hazardous substance or mixture under the GHS or the 2012 OSHA Hazard Communication Standard, and is intended for research use only.