Anti-Hu CD43, CD43-10G7, AZ, 25t

A mouse monoclonal antibody manufactured by STEMCELL Technologies, clone CD43-10G7, reacts with human CD43, a type I transmembrane sialoglycoprotein expressed on the surface of most human hematopoietic cells. Reported expression includes thymocytes, peripheral T cells, NK cells, monocytes, granulocytes, some B cells (activated B cells and an early B cell subset in bone marrow), plasma cells, and hematopoietic progenitor cells, with low expression observed on platelets. The antibody is of IgG1, kappa isotype, is verified for flow cytometry, and is supplied conjugated to APC.

CD43 exhibits different glycoforms in the range of approximately 95–135 kDa depending on cell type, binds ligands such as CD54 and CD169 (Siglec-1), and plays regulatory roles in cell adhesion and activation, apoptosis, and tumor progression. It serves as a marker for discriminating normal from neoplastic T cells and B cells within tissues, and binding of clone CD43-10G7 to CD43 on neutrophils reportedly induces homotypic cell adhesion. The reagent is supplied as a clear liquid and is not classified as a hazardous substance or mixture under the GHS or the 2012 OSHA Hazard Communication Standard, remaining stable under recommended transport and storage conditions.