Anti-Hu CD4, OKT4, AD, 25tests

This mouse monoclonal anti-human CD4 antibody (clone OKT4) is supplied by STEMCELL Technologies in the manufacturer's AD-conjugated format. CD4 is an ~59 kDa single-chain type 1 transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily, with four extracellular Ig-like domains (D1 – D4); the OKT4 epitope has been localized to the D3 domain.

The reagent is intended for flow cytometry-based immunophenotyping. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper cells), and at lower levels by peripheral blood monocytes and macrophages. CD4 binds to a non-polymorphic region of MHC II, acting as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition, and amplifies TCR signalling through its intracellular domain's interaction with kinases including Lck. CD4 is a receptor for the human immunodeficiency virus (HIV); binding of OKT4 to CD4 does not block HIV binding.

The clone has been verified for purity assessments of cells isolated with EasySep™ kits. The antibody is of mouse origin, isotype IgG2b, kappa. AD denotes a manufacturer-specific fluorochrome/code. Recommended storage is 2–8 °C; protect from light and avoid repeated freeze–thaw cycles.