Anti-Hu CD4, OKT4, AD, 100tests

CD4, an approximately 59 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily, is recognized by the Mouse monoclonal Anti-Human CD4 Antibody, Clone OKT4, from STEMCELL Technologies. The antibody's epitope is localized to the D3 domain of CD4, which contains four extracellular Ig-like domains (D1 – D4).

CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper cells), and at lower levels by peripheral blood monocytes and macrophages. It binds to a non-polymorphic region of MHC class II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition, enhancing the affinity of the TCR–MHC II–antigen complex association. CD4 also amplifies TCR signaling through interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck, and serves as a receptor for human immunodeficiency virus (HIV); binding of the OKT4 antibody to CD4 does not block HIV binding. The antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits.

The antibody is offered in multiple conjugate formats, including Alexa Fluor 488, APC, biotin, FITC, PE, PerCP-Cyanine5.5, and unconjugated. Reported applications include flow cytometry, functional assays, immunofluorescence, immunohistochemistry, and immunoprecipitation. The isotype is IgG2b, kappa, and the host species is mouse. Verified reactive species include human, chimpanzee, cynomolgus monkey, rhesus monkey, and white-necked white-browed monkey. Relevant cell types include T cells, CD4+ T cells, and regulatory T cells. The relevant research areas are immunology and transplantation research.