Anti-Hu CD326, VU-1D9, FI, 100tests

This FITC-conjugated monoclonal antibody from STEMCELL Technologies is a mouse IgG1, kappa reagent targeting human CD326 (EpCAM), also referred to as EGP2, ESA, TACSTD1, or TROP-1. Clone VU-1D9 was raised in BALB/c mice against the human small cell lung carcinoma cell line NCl-H69 and binds an epitope within the extracellular EGF-1 domain of EpCAM.

The target is an approximately 40 kDa type I transmembrane glycoprotein that acts as a homophilic, calcium-independent adhesion molecule with roles in signaling, migration, proliferation, and differentiation. EpCAM is expressed on the basolateral membrane of most normal epithelial cells — excluding nervous, muscular, and stromal tissue — and is highly expressed in bone marrow, colon, and lung. It appears early during erythrogenesis and is recognised as a stem cell marker. The protein is absent from mesothelial cells and mesotheliomas, supporting its routine use as a diagnostic marker to distinguish mesothelioma from carcinoma cells and as a target for immunotherapeutic treatment of carcinomas. Clone VU-1D9 has also been used to identify and isolate circulating tumor cells.

Verified and reported applications are limited to flow cytometry, where the suggested use is 20 μL per 1 x 10^6 cells in 100 μL. The reagent also performs on paraffin sections when a heat-induced antigen retrieval protocol is applied to formalin-fixed paraffin-embedded tissues. It is supplied in phosphate-buffered saline containing 0.1% bovine serum albumin and < 0.1% sodium azide and was purified by affinity chromatography. The antibody is stable at 2 - 8 °C when stored undiluted and should be protected from prolonged exposure to light; it must not be frozen. The product is for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.