Anti-Hu CD32, IV.3, FI, 25tests

From STEMCELL Technologies, this mouse monoclonal IgG2b, kappa antibody (Clone IV.3) targets human CD32 (also referred to as FcγRII or FCR II), an approximately 40 kDa type 1 transmembrane glycoprotein encoded by gene ID 2212. The reagent is FITC-conjugated and is supplied as a clear liquid. It is for research use only and is not intended for human or animal diagnostic or therapeutic uses.

The antibody was purified by affinity chromatography and formulated in phosphate-buffered saline containing less than 0.1% (w/v) sodium azide and less than 0.1% (w/v) bovine serum albumin. For flow cytometry, the suggested use is 20 μL per 1 x 10^6 cells in 100 μL, or per 100 μL of whole blood, with titration recommended for optimal performance. Verified applications include cell separation (CellSep) and flow cytometry (FC), and the IV.3 clone has been used as a blocking antibody to reduce non-specific binding in antibody-based detection and cell separation experiments. The antibody binds most strongly to the IIa isoforms of CD32, with the epitope mapped to amino acids 132 - 137 [FSHLDP] in domain 2, within the ligand binding site.

Product should be stored undiluted at 2 - 8°C; do not freeze and protect from prolonged exposure to light. STEMCELL Technologies' Quality Management System is certified to ISO 13485.