Anti-Hu CD3, OKT3, AZ, 100tests

STEMCELL Technologies offers a mouse monoclonal IgG2a, kappa antibody against human CD3, conjugated to APC (Allophycocyanin). The OKT3 antibody reacts with an extracellular conformational epitope on the ~20 kDa CD3ε subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. CD3 is a dimer assembled from combinations of CD3γ, δ, ε, η, and ζ subunits — structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily — that associates non-covalently with the TCR and is involved in transducing antigen recognition signals and in regulating T cell activation and TCR surface expression.

The target antigen is CD3 (alternative names: CD3 epsilon, CD3ε, Leu-4, T3; Gene ID: 916). Species reactivity is human, with the host species being mouse (CAF1). The immunogen was sheep erythrocyte rosette-purified human T cells. Clone OKT3 is widely used for phenotyping human T cells and has been observed to block the binding of anti-CD3 antibody clones SK7 and UCHT1; binding of OKT3 can induce T cell activation. Because of its immunosuppressive properties in vivo, OKT3 has proven useful in treating allograft rejection. The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions, and the solution is free of unconjugated APC. The reagent is formulated in phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide, 0.1% gelatin, and less than 0.2% (w/v) bovine serum albumin.

Applications verified and reported for this product are flow cytometry (FC). For flow cytometry, the suggested use is ≤ 5 μL per 1 x 10⁶ cells in 100 μL, and it is recommended that the antibody be titrated for optimal performance for each application. The product is stable at 2 - 8°C when stored undiluted; do not freeze and protect from prolonged exposure to light. Products are for research use only and are not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.