Anti-Hu CD235ab, HIR2, UC, 25µg

A primary antibody from STEMCELL Technologies, clone HIR2 (GA-R2), recognizes an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B). These are homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups, and are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation.

CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The HIR2 antibody binds with higher affinity to CD235a than CD235b and agglutinates untreated erythrocytes.

This unconjugated (UC) reagent is supplied alongside APC, biotin, FITC, and PE conjugations of the same clone. The antibody is a mouse IgG2b, kappa isotype and is suited to applications including flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, Western blot, and CyTOF, within the immunology area of research.