Anti-Hu CD235ab, HIR2, PE, 100µg

This monoclonal antibody from STEMCELL Technologies, clone HIR2 (also known as GA-R2), recognizes human CD235ab (Glycophorin A/B). It binds an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B), two homologous type I sialoglycoproteins on the erythrocyte membrane that carry the antigenic determinants for the MN and Ss blood groups.

CD235ab is expressed on early- and late-stage erythroblasts, mature red blood cells, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed while CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface that helps minimize erythrocyte aggregation in the circulation. The HIR2 antibody binds with higher affinity to CD235a than to CD235b and agglutinates untreated erythrocytes. The reagent has been verified for purity assessment of isolated cells.

The antibody is of mouse origin with an IgG2b, kappa isotype. Available conjugations include APC, biotin, FITC, PE, and unconjugated. Cited applications include CyTOF, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot, supporting research in immunology.