Anti-Hu CD235ab, HIR2, BT, 100µg

A biotin-conjugated monoclonal antibody from STEMCELL Technologies, this reagent targets human CD235ab (Glycophorin A/B) and is verified for flow cytometry. The clone HIR2 (also known as GA-R2) recognizes an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B), which are homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups.

CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation. The HIR2 antibody binds with higher affinity to CD235a than CD235b and agglutinates untreated erythrocytes.

The antibody is of mouse origin with IgG2b, kappa isotype, raised against a synthetic peptide corresponding to the N-terminal region of human CD235ab. It is supplied in an aqueous buffer containing 0.09% sodium azide and may contain carrier protein/stabilizer. For flow cytometry, the suggested use is ≤ 0.125 μg per 1 x 10^6 cells in 100 μL volume, with titration recommended for optimal performance. The product is stable at 2 - 8°C when stored undiluted; do not freeze. This antibody clone has been verified for purity assessments of cells isolated with EasySep kits, including the EasySep Human Positive Glycophorin A Depletion Cocktail and the RoboSep Human Positive Glycophorin A Depletion Kit.