Anti-Hu CD235ab, HIR2, AZ, 25µg

STEMCELL Technologies offers an antibody recognizing human CD235a (glycophorin A) and CD235b (glycophorin B), which are homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups. The HIR2 (GA-R2) antibody binds to an epitope common to the N-terminal region of both glycophorins, with higher affinity to CD235a than CD235b, and agglutinates untreated erythrocytes. CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types; CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation.

The antibody is of mouse origin with an IgG2b, kappa isotype, and reacts with human samples. Associated gene IDs are 2993/2994. It is suitable for applications including CyTOF, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot, supporting research in immunology. The antibody is available in multiple conjugations including APC, biotin, FITC, PE, and unconjugated formats.