Anti-Hu CD20, 2H7, PE, 100tests

A PE-conjugated mouse monoclonal antibody (clone 2H7, IgG2b kappa) from STEMCELL Technologies binds CD20, a non-glycosylated type I transmembrane protein in the MS4A family. The antibody recognizes an epitope mapped to the amino acid sequence YNCEPANPSEKNSPST, located in the large extracellular loop of a CD20 polypeptide that traverses the cell membrane four times. CD20 is expressed on pre-B cells, resting and activated B cells, some follicular dendritic cells, and a subset of T cells, with B-cell expression lost upon differentiation into plasma cells.

Reported species reactivity includes human, rhesus, cynomolgus, baboon, chimpanzee, capuchin monkey, Nancy Ma's night monkey, pigtailed macaque, and squirrel monkey. The reagent has been verified for cell separation (CellSep) and flow cytometry (FC) and has also been reported for FC. The antibody was raised against human tonsillar B cells and purified by affinity chromatography before conjugation with PE.

Supplied as a clear liquid in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, the product is stable at 2 - 8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. For flow cytometry, the suggested use is 5 μL per 1 x 10^6 cells in 100 μL, or per 100 μL of whole blood, with titration recommended for each application.