Anti-Hu CD2, RPA-2.10, BT, 100µg

Biotin-conjugated anti-human CD2 monoclonal antibody, clone RPA-2.10, mouse IgG1 kappa, is offered by STEMCELL Technologies for immunology research. Verified applications include cell separation, flow cytometry, functional assays, immunocytochemistry, immunofluorescence, immunohistochemistry, and Western blot. The antibody targets CD2 (Gene ID: 914), an approximately 50 kDa type I transmembrane glycoprotein of the immunoglobulin (Ig) superfamily that contains two Ig-like domains in its extracellular portion.

CD2 is broadly expressed on peripheral T cells, NK cells, dendritic cells, erythrocytes, most thymic T cells, subsets of thymic B cells, and on the endothelium. Its expression appears early during T cell differentiation, and aberrant expression has been observed in some lymphomas and myeloid leukemias. CD2 plays a critical role in T cell activation and signaling, as well as in lymphocyte adhesion. Its primary ligand is CD58 (LFA-3) on antigen-presenting cells, with additional ligands including CD15 (SSEA-1), CD48, and CD59. The RPA-2.10 clone has been reported to block the mixed lymphocyte reaction.

The biotin format is part of a series of available conjugations that also includes FITC, PE, APC, and unconjugated. The antibody has been verified for purity assessments of cells isolated with EasySep™ kits, including the HLA CD3 Positive Selection Kit, Human CD3 Positive Selection Kit, HLA Whole Blood CD3 Positive Selection Kit, and Human Whole Blood CD3 Positive Selection Kit. Reported reactive species include baboon, capuchin monkey, chimpanzee, crab-eating macaque (cynomolgus), human, rhesus monkey, pig, macaque, and squirrel monkey, supporting broad utility across non-human primate and human T cell research.