Anti-Hu CD184 (CXCR4), 12G5, PE, 25tests

A phycoerythrin (PE)-conjugated mouse monoclonal IgG2a, kappa antibody from STEMCELL Technologies, clone 12G5, targets human CD184 (also known as CXCR4 or fusin), an ~45 kDa seven-transmembrane G-protein-coupled receptor broadly expressed on T and B cells, dendritic cells, monocytes, macrophages, granulocytes, platelets, endothelial and epithelial cells, astrocytes, and neurons. Ligands for CD184 include the CXC chemokine SDF-1 and ubiquitin, and the receptor mediates chemotaxis, B-cell lymphopoiesis, myelopoiesis, cardiogenesis, vascularization, and neuronal guidance. CD184 also acts as a co-receptor for infection by some T-cell-tropic isolates of HIV-1 and as an alternative receptor for CD4-independent infection by HIV-2; binding of the 12G5 antibody blocks these viral entry functions and partially inhibits SDF-1-induced chemotaxis and calcium influx.

The antibody was raised in BALB/c mice using CP-MAC virus-infected SUP-T1 cells of human origin as the immunogen and shows reactivity with human, rhesus, cynomolgus, baboon, chimpanzee, African green monkey, and sooty mangabey samples. It has been verified for flow cytometry and is also reported for FACS and FC applications, and the clone has been validated for purity assessments of cells cultured with the STEMdiff™ Definitive Endoderm Kit, where CD184 is monitored alongside CD117 to track emergence of definitive endoderm. The reagent is supplied in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin; it was purified by affinity chromatography and conjugated with PE under optimal conditions, and is free of unconjugated PE and unconjugated antibody.

For flow cytometry, the suggested use is 5 μL per 1 x 10^6 cells in 100 μL, or per 100 μL of whole blood, with titration recommended for optimal performance for each application. The product is stable at 2 - 8°C when stored undiluted; it should not be frozen and must be protected from prolonged exposure to light. It is intended for research use only and not for human or animal diagnostic or therapeutic applications.