Anti-Hu CD184 (CXCR4), 12G5, AZ, 25tests

This APC-conjugated mouse monoclonal IgG2a, kappa antibody from STEMCELL Technologies, Clone 12G5, recognizes human CD184 (also known as CXCR4 or fusin), an ~45 kDa seven-transmembrane G-protein-coupled receptor expressed on the surface of a broad range of cells including T and B cells, dendritic cells, monocytes, macrophages, granulocytes, platelets, endothelial and epithelial cells, astrocytes, and neurons. The antibody has been verified for flow cytometry (FC) and shows cross-reactivity with rhesus, cynomolgus, baboon, chimpanzee, African green monkey, and sooty mangabey samples.

CD184 plays functional roles in chemotaxis, B cell lymphopoiesis and myelopoiesis, cardiogenesis, vascularization, and neuronal guidance. It also acts as a co-receptor for T cell-tropic isolates of HIV-1 and as an alternative receptor for CD4-independent infection by HIV-2 — functions that are blocked by binding of the 12G5 antibody, which additionally partially inhibits SDF-1-induced chemotaxis and calcium influx. In directed differentiation workflows, CD184 is used alongside markers such as CD117 to monitor the emergence of definitive endoderm using the STEMdiff™ Definitive Endoderm Kit.

The reagent is supplied in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, with a suggested use of 5 μL per 1 × 10^6 cells in 100 μL volume or per 100 μL of whole blood. It is produced in BALB/c mice using CP-MAC virus-infected SUP-T1 cells of human origin as the immunogen and is stable at 2 – 8 °C when stored undiluted; the product should not be frozen and should be protected from prolonged exposure to light.