Anti-Hu CD16, 3G8, BT, 25µg

A biotin-conjugated mouse monoclonal IgG1 antibody from STEMCELL Technologies targets human CD16 (FcγRIII), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. Designated Clone 3G8, the reagent was raised against human polymorphonuclear leukocytes and purified by affinity chromatography, with biotin conjugation carried out under optimal conditions; the resulting solution is free of unconjugated biotin.

CD16 exists in two isoforms: CD16a (50 - 65 kDa), expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils, and eosinophils and found as at least two polymorphic variants termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated, or complexed IgG, particularly the IgG1 and IgG3 isotypes, and engagement of either isoform triggers signaling that modulates antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and proliferation. Because CD16/IgG interactions can produce non-specific labeling in antibody-based detection and cell separation experiments, the 3G8 clone may be employed as a blocking antibody to reduce such binding, and it has been verified for use as a CD16 (FcγRIII receptor) blocking and/or labeling antibody with EasySep™ and RosetteSep™ Human kits.

The product is formulated in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide. It is verified for flow cytometry (FC) and reported for functional assay (FA) and immunohistochemistry (IHC). For flow cytometry, the suggested use is ≤ 1 μg per 1 x 10^6 cells in 100 μL volume or per 100 μL of whole blood, with titration recommended for optimal performance in each application. The product should be stored at 2 - 8°C undiluted and must not be frozen.