Anti-Hu CD14, M5E2, PE, 100tests

STEMCELL Technologies offers this mouse monoclonal IgG2a, kappa antibody, clone M5E2, PE-conjugated and directed against CD14. Raised against full-length human CD14 protein, the M5E2 clone reacts with CD14, an approximately 53–55 kDa GPI-anchored transmembrane glycoprotein expressed at high levels on peripheral blood monocytes and macrophages and at lower levels on granulocytes — an approximately 10-fold difference in expression levels that makes CD14 a useful marker for distinguishing these populations. CD14 is also found on tissue macrophages, Langerhans cells, and dendritic cells, and functions as a high-affinity receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, modulating LPS-dependent signal transduction during the immune response to gram-negative pathogens by acting as a co-receptor for TLR 4 and MD-2, triggering activation of NF-kappa-B, cytokine secretion, and the inflammatory response. Two soluble forms of CD14 (~48 and ~55 kDa) have also been described.

The antibody is reactive with human, rhesus, cynomolgus, chimpanzee, capuchin monkey, common marmoset, cotton-topped tamarin, pigtailed macaque, squirrel monkey, cow, dog, pig, and sheep. Flow cytometry is both verified and reported, and the clone has been used to assess purity of cells isolated with several EasySep™ kits. The reagent is supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin; it was purified by affinity chromatography and conjugated with PE. The product is stable at 2–8°C when stored undiluted; do not freeze, and protect from prolonged exposure to light. For flow cytometry, the suggested use is 5 μL per 1 x 10^6 cells in 100 μL volume or per 100 μL of whole blood.