Anti-Hu CD138, MI15, PE, 25tests

This mouse monoclonal IgG1 kappa antibody from STEMCELL Technologies, clone MI15, is conjugated to PE and recognizes CD138 (Syndecan-1), an approximately 85–95 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells), as well as on non-hematopoietic cells such as embryonic mesenchymal, endothelial, epithelial, and neural cells. CD138 expression is used as a diagnostic marker for several types of tumors, and CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration, and cell-matrix associations by linking the extracellular matrix to the cytoskeleton.

The antibody was raised against a combination of human-derived U266 and XG-1 myeloma cell lines, purified by affinity chromatography, and conjugated with PE under optimal conditions; the solution is free of unconjugated PE and unconjugated antibody. It is formulated as a phosphate-buffered solution at pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, and is supplied as a clear liquid. The clone reacts with human, rhesus, and cynomolgus (IHC) CD138.

Verified and reported applications include flow cytometry (FC), and this antibody clone has been verified for purity assessments of cells isolated with the EasySep™ Human CD138 Positive Selection Kit and the EasySep™ Human Whole Blood CD138 Positive Selection Kit. For flow cytometry, the suggested use is 5 μL per 1 × 10^6 cells in 100 μL volume or per 100 μL of whole blood; titration is recommended for optimal performance. The product is stable at 2 – 8°C when stored undiluted, should not be frozen, and should be protected from prolonged exposure to light. According to the safety data sheet, the product is not classified as hazardous under the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200).