Anti-Hu CD138, MI15, PE, 100tests

A mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies, PE-conjugated and directed against an extracellular epitope of CD138 (Syndecan-1), an ~85 - 95 kDa type 1 transmembrane glycoprotein. Clone MI15 recognizes CD138 expressed on pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells), as well as on non-hematopoietic cells including embryonic mesenchymal, endothelial, epithelial and neural cells. Reported species reactivity covers human, rhesus, and cynomolgus (IHC).

The antibody is formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, and was purified by affinity chromatography before PE conjugation under optimal conditions; the solution is free of unconjugated PE and unconjugated antibody. The immunogen was a combination of human-derived U266 and XG-1 myeloma cell lines. Flow cytometry has been verified for this clone, and a suggested use is 5 μL per 1 x 10^6 cells in 100 μL volume or per 100 μL of whole blood, with titration recommended for optimal performance. The clone has been verified for purity assessments of cells isolated with the EasySep™ Human CD138 Positive Selection Kit and the EasySep™ Human Whole Blood CD138 Positive Selection Kit. Store undiluted at 2 - 8°C; do not freeze, and protect from prolonged exposure to light.