Anti-Hu CD138, MI15, FI, 25tests

A FITC-conjugated mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies that targets CD138 (Syndecan-1), an ~85-95 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells), as well as on non-hematopoietic cells such as embryonic mesenchymal, endothelial, epithelial, and neural cells. The antibody, clone MI15, reacts with an extracellular epitope on CD138 and has been verified for flow cytometry (FC) applications, with IHC reported for rhesus and cynomolgus species reactivity. CD138 expression is used as a diagnostic marker for several types of tumors, and CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration, and cell-matrix associations by linking the extracellular matrix to the cytoskeleton.

The reagent is supplied as a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin. The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions; the solution is free of unconjugated FITC and unconjugated antibody. It is recommended for use as a laboratory reagent. For flow cytometry, the suggested use is 5 µL per 1 x 10^6 cells in 100 µL volume or per 100 µL of whole blood, and it is recommended that the antibody be titrated for optimal performance for each application.

Product is stable at 2 - 8°C when stored undiluted; do not freeze and protect from prolonged exposure to light. The product is classified as a clear liquid and is not a hazardous substance or mixture according to the Globally Harmonized System (GHS).