Anti-Hu CD138, MI15, 100ug

From STEMCELL Technologies, this mouse monoclonal antibody (clone MI15, isotype IgG1, kappa) targets an extracellular epitope on human, rhesus, and cynomolgus CD138 (Syndecan-1), an ~85 - 95 kDa type 1 transmembrane glycoprotein expressed on pre-B cells, immature B cells, and normal and malignant plasma cells, as well as on non-hematopoietic cells including embryonic mesenchymal, endothelial, epithelial, and neural cells. The reagent does not label mature circulating B cells.

CD138 is used as a diagnostic marker for several types of tumors and is thought to act primarily as a receptor that modulates cell proliferation, migration, and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. The MI15 clone recognizes a different epitope than the DL-101 anti-CD138 antibody but blocks binding of clone B-B4. The antibody was raised against a combination of human-derived U266 and XG-1 myeloma cell lines and purified by affinity chromatography. It is supplied unconjugated in a clear phosphate-buffered solution at pH 7.2, containing 0.09% sodium azide, and is intended for laboratory research use only.

The antibody has been verified for flow cytometry and reported for immunocytochemistry, immunofluorescence microscopy, immunohistochemistry, immunoprecipitation, and Western blotting. It has been verified for purity assessments of cells isolated with the EasySep Human CD138 Positive Selection Kit II and EasySep Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II. For flow cytometry, the suggested use is ≤ 0.5 μg per 1 x 10^6 cells in 100 μL or per 100 μL of whole blood. The product is stable at 2 - 8°C when stored undiluted; do not freeze. According to the manufacturer's Safety Data Sheet, the mixture is not classified as hazardous under GHS, with 0% of the mixture consisting of ingredient(s) of unknown toxicity, and the product is not regulated under DOT, TDG, MEX, ICAO, IATA, IMDG, RID, ADR, or ADN transport classifications.