Anti-Hu CD123, 6H6, PE, 100tests

A mouse monoclonal IgG1, kappa antibody from STEMCELL Technologies, clone 6H6, conjugated to PE (phycoerythrin), directed against human, rhesus, and sooty mangabey CD123 (IL-3 receptor subunit α). CD123 is described by the manufacturer as an ~70 kDa type I transmembrane glycoprotein belonging to the type I cytokine receptor family (type 5 subfamily) and the immunoglobulin superfamily, and it constitutes the ligand-binding α chain of the heterodimeric IL-3 receptor. On its own, CD123 binds IL-3 with low affinity, but achieves high-affinity binding when associated with CD131, the signal-transducing β chain of the receptor. The antibody has been verified for flow cytometry (FC) and is reported for FC, with suggested use at ≤ 5 μL per 1 × 10⁶ cells in 100 μL.

According to the manufacturer, CD123 is expressed by hematopoietic progenitor cells, endothelial cells, basophils, eosinophils, mast cells, monocytes, macrophages, dendritic cells, megakaryocytes, a subset of B cells, and by neutrophils when cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-3 binding to CD123 stimulates proliferation, differentiation, and viability of hematopoietic cells, and CD123 is noted as being highly expressed in malignancies such as acute leukemia. The 6H6 antibody does not inhibit binding of IL-3 to either CD123 or the IL-3 receptor. The reagent has been verified for purity assessments of cells isolated with EasySep™ kits, including the EasySep™ Human Plasmacytoid DC Enrichment Kit and the EasySep™ Human Basophil Enrichment Kit.

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions, and is supplied as a clear liquid. It is formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin, and is stable at 2 - 8 °C when stored undiluted. The product should not be frozen and should be protected from prolonged exposure to light.