Anti-Hu CD123, 6H6, AZ, 100tests

This reagent from STEMCELL Technologies is a mouse monoclonal IgG1 kappa antibody (clone 6H6) raised against human CD123, also known as IL-3 receptor subunit α. CD123 is an approximately 70 kDa type I transmembrane glycoprotein that belongs to the type I cytokine receptor family and the immunoglobulin superfamily, and it constitutes the ligand-binding α chain of the heterodimeric IL-3 receptor; when associated with CD131, it enables high-affinity binding of IL-3. The antibody was generated using COS cells transfected with a cDNA encoding the human IL-3Rα chain, and it does not inhibit binding of IL-3 to CD123 or the IL-3 receptor.

The APC (allophycocyanin)-conjugated reagent is supplied in a phosphate-buffered solution at pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) bovine serum albumin. It was purified by affinity chromatography and is free of unconjugated APC and unconjugated antibody. Clone 6H6 has been verified for flow cytometry, with a suggested use of ≤ 5 μL per 1 × 10⁶ cells in 100 μL, and has been used to assess the purity of cells isolated with the EasySep™ Human Plasmacytoid DC Enrichment Kit and EasySep™ Human Basophil Enrichment Kit.

Species reactivity includes human, rhesus, and sooty mangabey. CD123 is expressed on hematopoietic progenitor cells, endothelial cells, basophils, eosinophils, mast cells, monocytes, macrophages, dendritic cells, megakaryocytes, a subset of B cells, and on neutrophils cultured in the presence of GM-CSF; it is also reported to be highly expressed in malignancies such as acute leukemia. The product is stable at 2 – 8 °C when stored undiluted, should not be frozen, and should be protected from prolonged exposure to light. It is intended for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.