Anti-Hu Beta-Tubulin III, 2G10-TB3, 25µg

A mouse monoclonal IgG2a, kappa antibody (clone 2G10-TB3) from STEMCELL Technologies that recognizes beta-tubulin III, a structural cytoskeletal protein of the tubulin family. The antibody is unconjugated and reacts with human, mouse, rat, and other mammalian homologs. The epitope has reportedly been mapped to the extreme C-terminal amino acids EAQGPK, and the immunogen is a synthetic peptide corresponding to amino acids 436–450 of beta-tubulin III conjugated to keyhole limpet hemocyanin.

Verified applications include immunocytochemistry (ICC), immunofluorescence microscopy (IF), and western blotting (WB); reported applications additionally include flow cytometry (FC), immunohistochemistry (IHC), and immunoprecipitation (IP). For immunocytochemistry, a suggested use concentration of ≤ 5 µg/mL is provided, with titration recommended for optimal performance in each application. The clone has been verified for labeling neural stem and progenitor cells grown with STEMdiff™ Neural Induction Medium, STEMdiff™ Neural Progenitor Medium, NeuroCult™ NS-A Proliferation Kit (Human), NeuroCult™ Proliferation Kit (Mouse & Rat), and NeuroCult™ SM1 Neuronal Culture Kit.

Beta-tubulin III, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types. The product is supplied in an aqueous buffer containing 0.09% sodium azide, purified by column chromatography, and should be stored at 2–8 °C undiluted; do not freeze. For research use only and not intended for diagnostic or therapeutic use.