ACF Cell Dissociation Kit

An animal component-free enzymatic workflow from STEMCELL Technologies, the kit delivers gentle dissociation of cells from cultured monolayers and primary tissue specimens. The xeno-free formulation avoids exposure to bovine- or porcine-derived enzymes while preserving high cell viability yields, supporting downstream single-cell applications.

Researchers use this product for adherent cell passaging, dissociation of tumor and biopsy samples into single-cell suspensions, and preparation of cells for cell culture, flow cytometry analysis, and single-cell sequencing workflows. The defined-system chemistry supports reproducible results in translational and cell therapy research environments where animal-origin contaminants must be excluded.

The kit format includes multiple enzyme vials and buffer bottles arranged for routine bench-top use. Enzyme components are stored at -20 degrees C; accompanying buffers are stored at room temperature and protected from light where indicated. Each reaction requires a small sample volume in the microliter range, making the kit efficient for limited biopsy and primary specimens.

Applications extend across regenerative medicine, bioprocessing, and therapeutic cell development workflows where avoiding animal-origin contaminants is essential for downstream regulatory or clinical translation.