ZNF397 Colorimetric Cell-Based ELISA

Assay Genie's colorimetric in-cell ELISA kit is designed for the qualitative detection of ZNF397 protein expression directly in cultured cells, providing a lysate-free, high-throughput workflow. The kit is configured in a 96-well microplate format and is intended for use with adherent cell samples. With a stated dynamic range of greater than 5000 cells, it supports relative quantification of ZNF397 across treatment conditions, including screening the effects of siRNA, chemicals, or other activators and inhibitors on target levels.

Detection proceeds by an indirect ELISA format: target-specific primary antibodies capture ZNF397, and HRP-conjugated anti-rabbit or anti-mouse secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction at 450 nm after substrate addition. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalizing target absorbance values. A Crystal Violet whole-cell stain is also provided so that cell density can be determined and plating differences adjusted. The kit is stored at 4 °C with a stated stability of 6 months.

ZNF397 is a human transcription factor implicated in gene regulation and cell growth, and its dysregulation has been associated with cancer and neurological disorders. According to the included database annotations, the protein belongs to the krueppel C2H2-type zinc-finger family, with UniProt Q8NF99, Gene ID 84307, OMIM 609601 and chromosomal location 18q12.2; isoform 3 acts as a DNA-dependent transcriptional repressor. ZNF397 localises to the cytoplasm and nucleus and functions in metal ion binding, protein binding, sequence-specific DNA binding and transcription factor activity. The product is grouped under research in epigenetics and nuclear signaling.