ZNF265 Colorimetric Cell-Based ELISA

Assay Genie's ZNF265 Colorimetric Cell-Based ELISA is an in-cell immunoassay designed for the detection of ZNF265 protein expression profiles in cultured cells. The Cell-Based ELISA kit is intended for use with adherent cells and uses a qualitative, indirect ELISA format in which ZNF265 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies then catalyse a colour reaction upon substrate addition. The assay is described as lysate-free, high-throughput and sensitive, making it suitable for measuring relative ZNF265 levels in cultured cells and for screening the effects of various treatments, inhibitors such as siRNA or chemicals, and activators on ZNF265 expression.

Reactivity is reported for human, mouse and rat samples, with a colorimetric readout measured at 450 nm and a stated dynamic range of more than 5000 cells. The kit is supplied in a 96-Well Microplate format and is stored at 4°C with a stability of 6 months. Because the Cell-Based ELISA is qualitative, two normalisation approaches are included: a monoclonal anti-GAPDH antibody serves as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalised to cell number, adjusting for plating variation.

The target protein, ZNF265 (gene symbol ZRANB2, UniProt O95218), is described as a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts and as a zinc finger Ran-binding domain-containing protein with roles in RNA binding, transcription factor activity and zinc ion binding, with a reported molecular weight of 36,318 Da. The kit is categorised within the Epigenetics and Nuclear Signaling research area, supporting studies of RNA splicing, mRNA processing and DNA-dependent transcriptional regulation.