ZNF187 Colorimetric Cell-Based ELISA

This cell-based ELISA kit from Assay Genie is designed for the accurate detection of ZNF187 levels in cell lysates and tissue samples, with adherent cells as the relevant sample type. It offers high sensitivity and specificity for reliable and reproducible results across a variety of research applications. Detection is colorimetric, read at 450 nm, and the assay is specific for human samples with a stated dynamic range of > 5000 Cells. Storage and stability are specified at 4°C for 6 Months.

The kit is a convenient, lysate-free, high throughput and sensitive assay that can detect the ZNF187 protein expression profile in cultured cells. It can be used for measuring the relative amounts of ZNF187 in cultured cells, and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on ZNF187. Qualitative determination of ZNF187 concentration is achieved by an indirect ELISA format, in which ZNF187 is captured by ZNF187-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody; the conjugated HRP then catalyses a colorimetric reaction upon substrate addition. Because the assay is qualitative, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and the Crystal Violet whole-cell staining method can be used to determine cell density and adjust for plating differences.

According to the database information provided, ZNF187 (Gene ID: 7741, UniProt ID: Q16670) is a C2H2-type zinc finger protein that functions as a transcription factor, with documented molecular functions of protein binding, sequence-specific DNA binding, and transcription factor activity. It is localized to the cytoplasm, nucleoplasm, and nucleus, and the human ortholog maps to chromosomal location 6p21.31. The protein, also referred to as Zinc finger and SCAN domain-containing protein 26 (ZSCAN26), has a molecular weight of 37,560 Da and may be involved in transcriptional regulation. The kit is classified within the Epigenetics and Nuclear Signaling research area. ZNF187 is described as a key transcription factor that plays a critical role in regulating gene expression and cell function, with dysregulation of ZNF187 having been associated with cancer, autoimmune disorders, and developmental abnormalities.