ZNF134 Colorimetric Cell-Based ELISA

This cell-based ELISA kit from Assay Genie is designed for the qualitative determination of ZNF134 protein levels in cultured cells using an indirect ELISA format. Detection is colorimetric, with absorbance read at 450 nm, and the kit is reactive with human samples. It is intended for research in epigenetics and nuclear signaling.

ZNF134 is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody. HRP catalyses a colour reaction upon substrate addition. Because the assay is qualitative, two normalisation methods are provided: an anti-GAPDH monoclonal antibody serves as an internal positive control, and a Crystal Violet whole-cell stain can be used to determine cell density. The stated dynamic range supports analysis above 5000 cells, and storage is at 4 °C with a stated stability of 6 months. The kit can be used to measure relative ZNF134 expression in adherent cell cultures and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), and activators.

ZNF134 (zinc finger protein 134; UniProt P52741) is a krueppel C2H2-type zinc-finger protein that may be involved in transcriptional regulation, with two human isoforms produced by alternative splicing.