Zebrafish TNF alpha PharmaGenie ELISA Kit (SBRS1715)

The Zebrafish TNF alpha PharmaGenie ELISA Kit from Assay Genie provides quantitative measurement of tumor necrosis factor alpha in zebrafish-derived samples using a pre-coated microplate and a biotin-streptavidin signal amplification system. It is widely applied in developmental immunology, toxicology screening, and comparative cytokine studies that use zebrafish cell culture supernatants. Performance characteristics include a detection range of 15.6 to 1000 pg/mL and a minimum detectable dose of approximately 7.8 pg/mL, with 100 µL of sample loaded per well in duplicate. The capture antibody is zebrafish TNF alpha-specific, with no significant cross-reactivity observed against related mammalian or teleost cytokines such as IL-1 beta or IFN gamma. Unopened kit components are shipped refrigerated and should remain refrigerated until first use; reconstituted standards should be aliquoted and frozen to limit repeated freeze-thaw cycles. For research use only, not intended for diagnostic applications, this 96-determination format supports parallel evaluation of stimulation conditions, mutant lines, or chemical exposures.